【摘要】 为探究云南永德普洱生茶的品质及生物活性特征，采用高效液相色谱法HPLC和感官评价对永德普洱生茶的化学特征成分和品质进行分析，应用DPPH和ABTS⁺自由基清除和α-葡萄糖苷酶抑制模型测定抗氧化和降血糖活性。结果显示：永德普洱生茶的茶样中儿茶素C、表儿茶素EC、表没食子儿茶素EGC、表没食子儿茶素没食子酸脂EGCG、表儿茶素没食子酸脂ECG、茶棓素、大理茶素、咖啡因和茶氨酸的含量分别为0.46%～1.29%、1.45%～2.81%、0.97%～3.45%、4.13%～7.18%、4.01%～7.38%、1.62%～3.28%、0.31%～0.70%、2.71%～5.15%、1.14%～2.48%。同一乡镇的茶样其化学特征相似，崇岗茶样EGCG、茶棓素和大理茶素含量较高，德党茶样EC、咖啡因和茶氨酸含量较高，勐板茶样ECG、茶棓素、咖啡因和大理茶素含量较高。茶样感官品质优异，总评分数82.45～89.75。茶样的DPPH和ABTS⁺自由基清除IC₅₀分别为48.22～94.93μg·mL^-1和2.02～6.15μg·mL^-1,α-葡萄糖苷酶抑制IC₅₀为9.92～33.45μg·mL^-1。研究结果表明：云南永德普洱生茶的内含物丰富、口感醇厚，且其抗氧化和降血糖活性显著。更多还原

【Abstract】 To understand the quality and bioactivity of raw Pu-erh tea from Yongde county of Yunnan Province, the chemistry and quality of the tea samples were analyzed by high performance liquid chromatography technology combined with sensory evaluation, and the bioactivities were investigated by DPPH and ABTS⁺ free radical scavenging and α-glucosidase inhibition evaluation models.The results indicated that the contents of C,EC,EGC,EGCG,ECG,theogallin, talinin, caffeine and theanine were 0.46%-1.29%,1.45%-2.81%,0.97%-3.45%,4.13%-7.18%,4.01%-7.38%,1.62%-3.28%,0.31%-0.70%,2.71%-5.15%,and 1.14%-2.48%,respectively, in the tea samples.There were similar chemical characteristics among tea samples from the same township.For example, contents of EGCG,theogallin, and talinin were higher in Chonggang tea samples; contents of EC,caffeine, and theanine were higher in Dedang tea samples; contents of ECG,theogallin, caffeine, and talinin were higher in Mengban tea samples.The sensory quality of Yongde raw Pu-erh tea is excellent, with total scores ranging from 82.45 to 89.75.The results of the activity evaluation of the tea samples suggested that the IC₅₀values for DPPH and ABTS⁺ free radical scavenging were 48.22-94.93 μg·mL^-1and 2.02-6.15 μg·mL^-1,respectively, and the IC₅₀ value for α-glucosidase inhibition was 9.92-33.45 μg·mL^-1.The research findings show that raw Pu-erh tea from Yongde, Yunnan, is rich in intrinsic compounds, offers a mellow taste, and exhibits significant antioxidant and hypoglycemic activities.更多还原