【摘要】 本文利用3-氧代-5β-24-胆烷酸甲酯（4）与单缩二氨基硫脲（2）为原料,合成了一种新型甾体不对称双缩二氨基硫脲席夫碱荧光探针N′-（2-羟基亚苄基）-2-（3-基亚甲基-5β-24-胆烷酸甲酯）肼基-1-硫代碳酰肼（L）。在缓冲溶液（pH 6～9）中,探针L可以高选择性荧光识别Zn²⁺,检测限可达到0.21μM。在365 nm紫外灯照射下,溶液由无色变成亮绿色强荧光。生成1∶1型配合物(L-Zn²⁺)。实际应用研究表明,探针L可应用于实际水样中一定浓度范围内Zn²⁺的检测。更多还原

【Abstract】 A novel asymmetric bis-Schiff base fluorescent probe methyl（5β）-3-[2-[[（2-hydroxyphenylideneamin）-amino]thioxomethyl]hydrazinylidene]-cholan-24-oate（L）was synthesized by using methyl（5β）-3-oxocholan-24-oate and 2-hydroxybenzaldehyde thiocarbohydrazone as starting materials.In an ethanol/tris（pH 6～9）buffer solution,the probe L displayed excellent chemo-selectivity towards Zn²⁺ ions,the limit of detection of the probe L for Zn²⁺ could reach 0.21 μM.When Zn²⁺ was coordinated with probe L,a 1∶1 type complex(L-Zn²⁺)formed and fluorescence color of the system was changed from non-fluoresce to strong bright-green fluorescence under 365 nm UV lamp irradiation.Furthermore,the proposed research revealed that the probe L could be used for the detection of Zn²⁺ in actual water samples.更多还原